Guidelines are available for euthanasia procedures Durante animals,1 including with IV and intraorgan administration of a pentobarbital anesthetic. It is imperative that any euthanasia technique deliver a pain- and stress-free death.1 Different euthanasia techniques can result Con shorter or longer times to death depending on the absorption rate of pentobarbital Con the body and, ultimately, the brain (Table).
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2007. Implications of experimental technique for analysis and interpretation of patronato from animal experiments: outliers and increased variability resulting from failure of intraperitoneal injection procedures. Lab Anim
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And while the Companion Animal Euthanasia Training Academy (CAETA) is an advocate for intraorgan injections as viable technique options, they do require more solution, sometimes triple the IV porzione. Con a time of shortage, IV administration will help to preserve what minimal drug reserves there are.
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The stressful nature of intraperitoneal injections has been inferred strada the measurement of hormonal markers. Per mezzo di one study, ACTH levels increased as much as 2-fold compared with basal levels Durante Sprague–Dawley rats after intraperitoneal injections of saline.33 However, the concentrations of ACTH measured varied substantially. A similar increase was observed Per mezzo di mice.
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Two studies examined the position of the cecum Con rats and mice and found that it was predominantly Sopra the left caudal abdominal quadrant.28,98 This finding is consistent with the practice of giving injections in the right caudal quadrant to avoid penetrating the cecum. However, the lateral dominance of the organ never exceeded 80%. Moreover, the percentage varied among strains, sexes, and even colonies.
When working with alternatives, veterinarians and other practitioners may feel compelled to prepare witnesses for physiologic variations compared to pentobarbital-induced death. This is reasonable, however, detailed descriptions may not be necessary. Each of the following alternatives require patient unconsciousness before administration, therefore any and all active signs of death go unperceived by the patient.